Determination of cannabinoids in plasma using salting‐out assisted liquid‐liquid extraction followed by LC‐MS/MS analysis
C.P. Da Silva L.P.P. Dalpiaz F.E. Gerbase V.V. Muller A. Cezimbra da Silva L.L. Lizot R. Hahn J.L. Costa M.V. Antunes R. Linden.
Abstract
The detection of the markers of Cannabis consumption in biological specimens is an important task for drug testing laboratories in different contexts. A simple assay combining salting-out assisted liquid-liquid extraction (SALLE) sample preparation and LC-MS/MS analysis was applied to the measurement of THC, THC-COOH, 11-OH-THC, CBN and CBD concentrations in 100 µL plasma specimens. The assay had linearity between 1-100 ng mL -1 for THC-COOH and 0.5 to 50 ng mL -1 for the other tested cannabinoids. Assay validation criteria were fulfilled. Extraction yields (88.7-97.3%) and internal- standard correct matrix effects (-9.6 to +5.4%) were acceptable. The assay was applied to 238 clinical specimens from trauma patients, with 19 samples presenting quantifiable concentrations of at least one of the target compounds. The developed assay is a simple and efficient strategy for simultaneous measurement of THC, THC-COOH, 11-THC-OH, CBN and CBD concentrations in plasma specimens.
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